As the number of US adult Twitter users is projected to increase by 7.1 million users by 2013 (eMarketer 2011), this microblogging site is becoming an increasingly viable method of professional networking and information sharing. Presenters will discuss the potential roles of Twitter in public health communications and networking, particularly in fostering engagement and collaboration via different forms of Twitter Chats. Twitter Chats are interactive online discussions where participants post messages with a focus on a specific topic for a set period of time on Twitter. The presentation will include best practices and strategies for hosting and participating in Twitter Chats, and their potential impact on a community, with fleshed out case studies of using Twitter chats for a specific cause, including health literacy and HIV testing. Twitter Chats are a valuable and cost-effective way to bring a diverse group of professionals together to discuss important health issues, and the tools used to implement them can be tailored to an organization’s size, resources and objectives. The interactive nature of these chats also supports a collaborative communication model, supporting a shift away from traditional one-way communication. The presenters explore how this collaborative paradigm can be leveraged using different strategies and tools of engagement through Twitter.